Journal: Laboratory animal research
Article Title: Observation of neutrophil extracellular traps in the development of diabetic nephropathy using diabetic murine models.
doi: 10.1186/s42826-024-00226-2
Figure Lengend Snippet: Fig. 4 Apoptosis and viability with NET or NET blocking in HK2 cells. (A) Annexin V-FITC and propidium iodide (PI) staining and (B) quantitative analyses for apoptosis after co-culture of neutrophils in HK2 cells. n = 4 each group. (C) MTT assay results according to NET blocking in HK2, CIHGM-1, and ciGEnC cells. n = 8 each group. (One-way ANOVA, ***p < 0.001 vs. con, ## p < 0.01, p < 0.001 vs. 20:1)
Article Snippet: We purchased Human Kidney-2 Cell Line (HK2) from the American Type Culture Collection (Virginia, USA).
Techniques: Blocking Assay, Staining, Co-Culture Assay, MTT Assay